Human PPM1G ORF mammalian expression plasmid, C-Flag tagRegisterSign inHiSignoutMy AccountBulk OrderCart (0)Contact UsAfter search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us！ProteinAntibodyGene/cDNAqPCR PrimerELISA kitsToolsSite Search by googleAllRecombinant ProteinAntibodyELISA Pair SetcDNA ORF CloneELISA KitqPCRotherProduct SearchEnglishEnglishChinese (中文简体)Japanese (日本语)German (Deutsch)French (Francais)Korean (한국어)Spanish (Español)Portuguese (português)Russian (русский)Chinese (中文繁體)Product TypeQuick OrderAdd to CartProductsRecombinant Proteins▷ Human Protein▷ Mouse Protein▷ Rat Protein▷ Cynomolgus Protein▷ Canine Protein▷ Influenza Protein▷ Virus Protein▷ Other ProteinAntibody▷ Primary Antibody
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BGH(TAGAAGGCACAGTCGAGG)Promoter:Enhanced CMV mammalian cell promoterApplication:Stable or Transient mammalian expressionAntibiotic in E.coli:Kanamycin Antibiotic in mammalian cell:HygromycinShipping_carrier:Each tube contains lyophilized plasmid.Storage:The lyophilized plasmid can be stored at room temperature for three months.PPM1Gneucleotide sequence and amino acid sequence pCMV3-C-FLAG Vector Information
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive. Human PPM1G ORF mammalian expression plasmid, C-Flag tag on other vectors Human PPM1G ORF mammalian expression plasmid, C-GFPSpark tagHG11245-ACG$345Human PPM1G ORF mammalian expression plasmid, C-OFPSpark / RFP tagHG11245-ACR$345Human PPM1G ORF mammalian expression plasmid, N-GFPSpark tagHG11245-ANG$345Human PPM1G ORF mammalian expression plasmid, N-OFPSpark / RFP tagHG11245-ANR$345Human PPM1G ORF mammalian expression plasmid, C-Flag tagHG11245-CF$315Human PPM1G ORF mammalian expression plasmid, C-His tagHG11245-CH$315Human PPM1G ORF mammalian expression plasmid, C-Myc tagHG11245-CM$315Human PPM1G ORF mammalian expression plasmid, C-HA tagHG11245-CY$315Human PPM1G Gene cDNA clone plasmidHG11245-M$115Human PPM1G ORF mammalian expression plasmid, N-Flag tagHG11245-NF$315Human PPM1G ORF mammalian expression plasmid, N-His tagHG11245-NH$315Human PPM1G ORF mammalian expression plasmid, N-Myc tagHG11245-NM$315Human PPM1G ORF mammalian expression plasmid, N-HA tagHG11245-NY$315Human PPM1G natural ORF mammalian expression plasmidHG11245-UT$315 Learn more about expression Vectors Proteins Antibodies ELISA KitsProduct nameProduct nameProduct nameProduct nameProduct nameProduct nameBackgroundProtein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma, PPM1G and PPM1C, is a cytoplasm protein which belongs to the PP2C family. PPM1G / PP2C-gamma is widely expressed. It is most abundant in testis, skeletal muscle, and heart. Alternatively spliced transcript variants encoding the same protein have been described. PP2C family members are known to be negative regulators of cell stress response pathways. PPM1G / PP2C-gamma is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. PPM1G / PP2C-gamma also plays a role in regulating cell cycle progression.References
Size / PriceCatalog: HG11245-CFList Price: (Save )Price: [How to order]Availability2-3 weeksShipping instructionsSize:Quantity:Add to CartBulk Discount InquiryImagesRecently Viewed ItemsHuman PPM1G ORF mammalian expression plasmid, C-Flag tagRecommended ProductsSinofection Transfection ReagentPlease note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"ProductsBy TypesProteinAntibodyELISA KitcDNA CloneTransfection ReagentRecombinant EnzymesAntibody PurificationAntibody Isotyping KitsBy Research TopicsCancerDevelopmental BiologyEnzymeEpigeneticsImmunologyNeuroscienceSignal TransductionStem CellServiceAntibody Development & ProductionProtein Expression & ProductionHybridoma One-Stop ServicesStable Cell Line DevelopmentBioanalytical Assay ServicesHelp & SupportHow to OrderPayment InstructionShipping InformationTechnical FAQsLeave Us a MessageContact Usorder@sinobiological.com+86-400-890-9989Follow Sino Biological on:微信服务号：义翘神州生物TermsPrivacy PolicyLegalSitemapFriends links© 2007-2015 Sino Biological Inc. All rights reservedTop
Human PPM1G ORF mammalian expression plasmid, C-Flag tagRegisterSign inHiSignoutMy AccountBulk OrderCart (0)Contact UsAfter search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us！ProteinAntibodyGene/cDNAqPCR PrimerELISA kitsToolsSite Search by googleAllRecombinant ProteinAntibodyELISA Pair SetcDNA ORF CloneELISA KitqPCRotherProduct SearchEnglishEnglishChinese (中文简体)Japanese (日本语)German (Deutsch)French (Francais)Korean (한국어)Spanish (Español)Portuguese (português)Russian (русский)Chinese (中文繁體)Product TypeQuick OrderAdd to CartProductsRecombinant Proteins▷ Human Protein▷ Mouse Protein▷ Rat Protein▷ Cynomolgus Protein▷ Canine Protein▷ Influenza Protein▷ Virus Protein▷ Other ProteinAntibody▷ Primary Antibody
▪ Protein Production by E. coli. fermentation▷ Protein Refolding Service▷ His-tag Protein Production Service▷ Flag-tag Protein Production Service▷ Fc-fusion-tag Protein Production Service▷ HA-tag Protein Production Service▷ GST-fusion-tag Protein Production Service▷ Myc-tag Protein Production ServiceAntibody Production & Development Service▷ Antibody Production CRO Service▷ Antibody Purification CRO Service▷ Transient Transfection Antibody Production Service▷ Custom Antibody Development CRO Service▷ Antibody Humanization Engineering CRO Service▷ Antibody Affinity Purification Resin / Argarose Beads Transient Transfection CRO Service▷ Transient Transfection Technology in HEK293 Cells▷ Transient Transfection Technology in CHO cells▷ Transient Transfection Service for Antibody Production▷ Transient Transfection Service for Protein Production▷ Affordable Large scale transfection reagent: SinofectionMolecular Biology Service▷ Gene Cloning CRO Service▷ Gene Synthesis CRO Service▷ Expression-Ready Vector Construction CRO Service▷ Plasmid Production CRO Service▷ Real Time qPCR CRO Service CRISPR/Cas9 Service▷ gRNA Design and Vector Construction▷ Gene Knock-out Cell Lines Construction▷ Gene Knock-in Cell Lines Construction SupportHow to order▷ Online Order▷ Purchase Order (PO)▷ How to Pay?▷ Shipping Information▷ Ordering FAQHow to contact us▷ Telephone & email▷ Scientific Meetings▷ Leave a Message▷ Distributors▷ Bulk order inqueryTechnical FAQAbout UsOur historyQualityLeadership teamCitationsCompany newsCustomer feedbackExhibition informationDownload centerProduct advantagesContact usDistributorsAustriaUrkraineDenmarkEstoniaFranceBelgiumBelarusBrazilAustraliaHongkongIrelandIcelandIndiaIsraelCanadaFinlandGermanyCzech RepublicMacauNorwayRussiaSingaporeSlovakiaSpainJapanItalyLuxembourgKoreaKazakhstanLatviaLithuaniaSwedenSwitzerlandTaiwanU.KChinaThe web search service will be supported BY google.Don't worry. This action will let you stay in our website.Home>Cancer>Signal transduction>Protein Phosphorylation>Serine / threonine Phosphatase>PPM1GText Size:AAAcdna-cloneppm1gHG11245-CFHuman PPM1G ORF mammalian expression plasmid, C-Flag tagSubmit Review | Submit ReferenceLeave a Message Add to favoriteAuthorDateRatingApplicationSpeciesImageDetails0DatasheetSpecific ReferencesReviewsRelated ProductsProtocolsHuman PPM1G cDNA Clone Product InformationGene_bank_ref_id:NM_177983.1RefSeq ORF Size:1641bpcDNA Description:Full length Clone DNA of Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent, 1G with C terminal Flag tag.Gene Synonym:PP2CG, PPP2CG, MGC1675, MGC2870, PP2CGAMMA, PPM1GSpecies:HumanVector:pCMV3-C-FLAGPlasmid:Restriction Site:Tag Sequence:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAGSequence Description:Sequencing primers:T7(TAATACGACTCACTATAGGG)
BGH(TAGAAGGCACAGTCGAGG)Promoter:Enhanced CMV mammalian cell promoterApplication:Stable or Transient mammalian expressionAntibiotic in E.coli:Kanamycin Antibiotic in mammalian cell:HygromycinShipping_carrier:Each tube contains lyophilized plasmid.Storage:The lyophilized plasmid can be stored at room temperature for three months.PPM1Gneucleotide sequence and amino acid sequence pCMV3-C-FLAG Vector Information
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It c
an be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive. Human PPM1G ORF mammalian expression plasmid, C-Flag tag on other vectors Human PPM1G ORF mammalian expression plasmid, C-GFPSpark tagHG11245-ACG$345Human PPM1G ORF mammalian expression plasmid, C-OFPSpark / RFP tagHG11245-ACR$345Human PPM1G ORF mammalian expression plasmid, N-GFPSpark tagHG11245-ANG$345Human PPM1G ORF mammalian expression plasmid, N-OFPSpark / RFP tagHG11245-ANR$345Human PPM1G ORF mammalian expression plasmid, C-Flag tagHG11245-CF$315Human PPM1G ORF mammalian expression plasmid, C-His tagHG11245-CH$315Human PPM1G ORF mammalian expression plasmid, C-Myc tagHG11245-CM$315Human PPM1G ORF mammalian expression plasmid, C-HA tagHG11245-CY$315Human PPM1G Gene cDNA clone plasmidHG11245-M$115Human PPM1G ORF mammalian expression plasmid, N-Flag tagHG11245-NF$315Human PPM1G ORF mammalian expression plasmid, N-His tagHG11245-NH$315Human PPM1G ORF mammalian expression plasmid, N-Myc tagHG11245-NM$315Human PPM1G ORF mammalian expression plasmid, N-HA tagHG11245-NY$315Human PPM1G natural ORF mammalian expression plasmidHG11245-UT$315 Learn more about expression Vectors Proteins Antibodies ELISA KitsProduct nameProduct nameProduct nameProduct nameProduct nameProduct nameBackgroundProtein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma, PPM1G and PPM1C, is a cytoplasm protein which belongs to the PP2C family. PPM1G / PP2C-gamma is widely expressed. It is most abundant in testis, skeletal muscle, and heart. Alternatively spliced transcript variants encoding the same protein have been described. PP2C family members are known to be negative regulators of cell stress response pathways. PPM1G / PP2C-gamma is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. PPM1G / PP2C-gamma also plays a role in regulating cell cycle progression.References
Size / PriceCatalog: HG11245-CFList Price: (Save )Price: [How to order]Availability2-3 weeksShipping instructionsSize:Quantity:Add to CartBulk Discount InquiryImagesRecently Viewed ItemsHuman PPM1G ORF mammalian expression plasmid, C-Flag tagRecommended ProductsSinofection Transfection ReagentPlease note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"ProductsBy TypesProteinAntibodyELISA KitcDNA CloneTransfection ReagentRecombinant EnzymesAntibody PurificationAntibody Isotyping KitsBy Research TopicsCancerDevelopmental BiologyEnzymeEpigeneticsImmunologyNeuroscienceSignal TransductionStem CellServiceAntibody Development & ProductionProtein Expression & ProductionHybridoma One-Stop ServicesStable Cell Line DevelopmentBioanalytical Assay ServicesHelp & SupportHow to OrderPayment InstructionShipping InformationTechnical FAQsLeave Us a MessageContact Usorder@sinobiological.com+86-400-890-9989Follow Sino Biological on:微信服务号：义翘神州生物TermsPrivacy PolicyLegalSitemapFriends links© 2007-2015 Sino Biological Inc. All rights reservedTop